investigation of the active biofilm communities on


reflected in the active biofilm communities which shifted from being Clostridiales dominated Site Investigation At the interface between the alkaline leachate and the surrounding land a series of 2 2 cm boreholes were emplaced to an approximate depth of 0 5 m at all three sites These boreholes were encased with plastic liners that were perforated for the bottom 5 cm Nylon mesh bags

Quantification of Electron Transfer Rates to a Solid

Microbial fuel cell (MFC) technology has enabled new insights into the mechanisms of electron transfer from dissimilatory metal reducing bacteria to a solid phase electron acceptor Using solid electrodes as electron acceptors enables quantitative real-time measurements of electron transfer rates to these surfaces We describe here an optically accessible dual anode continuous flow MFC that

Dissimilatory Iron Reduction and Odor Indicator

Dissimilatory Iron Reduction and Odor Indicator Abatement by Biofilm Communities in Swine Manure Microcosms DNA sequences obtained from rRNA gene amplicons of bacterial communities in FeCl3-treated microcosms were closely related to Desulfitobacterium spp which are known representatives of DIRB Use of iron respiration to abate wastewater odors warrants further investigation

An in vitro biofilm model system maintaining

Meeting the challenges with oral in vitromodel systems The goal of this study was to develop and validate with deep sequencing a novel robust in vitro model system representative of the naturally complex oral microbiome In previous studies of biodiverse model biofilm systems a major approach has been to construct synthetic plaque-like consortia represented by a handful of plaque species [18

Development and Characterization of an In Vivo Central

Biofilms represent a niche for microorganisms where they are protected from both the host immune system and antimicrobial therapies Biofilm growth serves as an increasing source of clinical infections Candida infections are difficult to manage due to their persistent nature and associated drug resistance Observations made in biofilm research have generally been limited to in vitro models

Mechanisms of biofilm resistance to antimicrobial

Biofilms are communities of microorganisms attached to a surface It has become clear that biofilm-grown cells express properties distinct from planktonic cells one of which is an increased resistance to antimicrobial agents Recent work has indicated that slow growth and/or induction of an rpoS-mediated stress response could contribute to biocide resistance

Protozoan grazing and its impact upon

Protozoan grazing and its impact upon population dynamics in biofilm communities Huws S A McBain A J Gilbert P 2005-01-01 00:00:00 Aims: To determine the impact of protozoan grazing on the population dynamics of a multispecies bacterial biofilm community Methods and Results: Grazing by Acanthamoeba castellanii and the ciliate Colpoda maupasi upon biofilm and planktonic communities

Bacterial Communities and Biofilms – Wong Lab

Bacterial Communities and Biofilms Search this website Home Research Areas Bacterial Communities and Biofilms Biofilms are surface-associated socially-organized bacterial communities Biofilm infections occur in a broad range of contexts (in the lung and essentially any biomedical implant) and are notoriously difficult to treat with antibiotics due to biofilm-related tolerance For

Options and Limitations in Clinical Investigation of

04 04 2018INTRODUCTION Biofilms are multidimensional communities in which resident bacteria coexist within the self-derived extracellular matrix (ECM) (1 2) Although the developmental stages leading to biofilm formation appear to be conserved (Fig 1) every species (or consortium of species in the case of polymicrobial biofilms) forms a unique multicellular community (3 4)

Staphylococcus aureus biofilms

investigation Although a biofilm can arise from a single cell differential environmental conditions throughout the community can poten - tiate the development of distinct subpopulations Gradients in oxygen nutrients and electron acceptors can cause heterogeneous gene expression throughout a biofilm 4 In a staphylococci in vitro colony biofilm model four distinct metabolic states were

Examining the Relationship Between Iron Corrosion and

Hyun-Jung Jang Young-June Choi Hee-Myong Ro Jong-Ok Ka Effects of phosphate addition on biofilm bacterial communities and water quality in annular reactors equipped with stainless steel and ductile cast iron pipes The Journal of Microbiology 10 1007/s12275-012-1040-x 50 1 (17-28) (2012)

Microbial biofilm inhibits wound healing

This investigation of biofilm formation in chronic wounds will place a special focus on the opportunistic pathogen responsible for increasing resistance to antibiotics in biofilm communities When P aeruginosa was treated with furanone and tobramycin shutting down the QS system caused the bacteria to become more sensitive to the antibiotic (Figure 8) The QS blocker itself did not weaken


Bacterial biofilms are communities of surface-associated microorganisms living in cellular clusters or micro-colonies encapsulated in a complex matrix composed of an extracellular polymeric substance separated by open water channels that act as a circulatory system that enable better diffusion of nutrients and easier removal of metabolic waste products

Disrupting the biofilm matrix improves wound healing

Debridement has been a mainstay in disrupting biofilm and forcing biofilm to reconstitute itself 15 Post debridement when the biofilm microbes are disorganised and inadequately protected by the disrupted matrix they are forced to become metabolically active to reconstitute the matrix becoming much more vulnerable to standard treatments such as antiseptics biocides and antibiotics 16

Center for Biofilm Engineering (CBE)

At the Center for Biofilm Engineering (CBE) multidisciplinary research teams develop beneficial uses for microbial biofilms and find solutions to industrially relevant biofilm problems The CBE was established at Montana State University Bozeman in 1990 as a National Science Foundation Engineering Research Center As part of the MSU College of Engineering the CBE gives students a chance to

Investigation of microbial biofilm structure by laser

Investigation of microbial biofilm structure by laser scanning microscopy Coronavirus: Find the latest articles and preprints Microbial communities associated with interfaces and the samples thereof may come from natural technical and medical habitats For imaging such complex microbial communities confocal laser scanning microscopy (CLSM) is the method of choice CLSM allows

Staff View: Biofilms

|t Cell Biomass Determination in Sewer Biofilms and a Monospecies Pseudomonas Putida Biofilm / |r Andreas Jahn and Per Halkjaer Nielsen -- |g 6 |t Simultaneous Morphological and Population Analyses of Environmental Biofilms / |r Jannie A Flood Nicholas J Ashbolt and Peter J Beatson -- |g 7


Abundance and diversity of microbial communities in long-term operated anammox biofilm reactors initiated with different inocula In: 5th IWA International Specialized Conference on Microbial Population Dynamics in Biological Wastewater Treatment Aalborg Denmark [Conference item]

Potential for hydrogen

Potential for hydrogen-oxidizing chemolithoautotrophic and diazotrophic populations to initiate biofilm formation in oligotrophic deep terrestrial subsurface waters Xiaofen Wu 1 Karsten Pedersen 2 Johanna Edlund 2 Lena Eriksson 2 Mats strm 3 Anders F Andersson 4 Stefan Bertilsson 5 Mark Dopson 1 Microbiome volume 5 Article number: 37 (2017) Cite this article 2572 Accesses 14

Structural and functional diversity of biofilm bacterial

The alpha diversity of biofilm bacterial communities measured by the Shannon and Chao 1 indices generally decreased along the Pearl River Estuary with minimal values of both occurring at NP The beta-diversity of the biofilm bacterial community was determined by

Biofilm Formation as Microbial Development

Biofilms can be defined as communities of microorganisms attached to a surface It is clear that microorganisms undergo profound changes during their transition from planktonic (free-swimming) organisms to cells that are part of a complex surface-attached community These changes are reflected in the new phenotypic characteristics developed by biofilm bacteria and occur in response to a

Biofilm Resistance to Antimicrobials

These microbial biofilm communities are noticeably resistant to and difficult to eradicate with antimicrobials The increased tolerance and resistance of biofilm cells compared to planktonics are thought to be regulated by a number of mechanisms including slow penetration of the antimicrobial agent through the biofilm changes in the chemical microenvironment within the biofilm (leading to

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