rapid isolation and profiling of a diverse panel of human

Activity

Activity-based protein profiling (ABPP) has emerged as a powerful chemical proteomic strategy to characterize enzyme function directly in native biological systems on a global scale Here we review the basic technology of ABPP the enzyme classes addressable by this method and the biological discoveries attributable to its application Key Words affinity label mass spectrometry proteomics

Analytical Validation of a Hybrid Capture–Based Next

Genomic profiling of circulating tumor DNA derived from cell-free DNA (cfDNA) in blood can provide a noninvasive method for detecting genomic biomarkers to guide clinical decision making for cancer patients We developed a hybrid capture–based next-generation sequencing assay for genomic profiling of circulating tumor DNA from blood (FoundationACT)

Identification isolation and characterization of human

Isolation culture and genomic characterization of human adenoma organoids We have developed an ongoing repository of organoids from patient-derived adenomas (n=17 including two high-risk sessile serrated adenomas) adenocarcinomas (n=4 including one colitis-associated cancer) and normal colon (n=9) All have been cryopreserved at early passage reestablished from frozen stock validated as

Bioassay

Hyaluronidase enzyme (HAase) has a role in the dissolution or disintegration of hyaluronic acid (HA) and in maintaining the heathy state of skin Bioassay-guided fractionation of Ravenala madagascariensis (Sonn ) organ extracts (leaf flower stem and root) testing for hyaluronidase inhibition was performed followed by metabolic profiling using LCndash S

A novel human gastric primary cell culture system for

Confocal microscopy of human gastric organoids shows that—similar to spheroids—they consist of a single layer of cylindrical highly polarised epithelial cells as indicated by E-cadherin and β-catenin immunolabelling (figure 3D upper panel) In contrast to the perfectly rounded spheroids the organoids exhibit folded gland-like structures typically found in healthy gastric mucosa

Probe

Probe-Seq allowed the isolation and profiling of RNA from fresh mouse frozen human and fresh chick retinas as well as gut cells from Drosophila melanogaster Aside from the different dissociation protocols Probe-Seq does not require species- or tissue-specific alterations To profile multiple cellular subtypes multiplexed Probe-Seq allows for iterative labeling sorting and re-labeling

A PEROXO

Peroxisomes are metabolic organelles that perform a diverse array of critical functions in human physiology Traditional isolation methods for peroxisomes can TongWei more than 1 h to complete and can be laborious to implement To address this we have now extended our prior work on rapid organellar isolation to peroxisomes via the development of a peroxisomally localized 3XHA epitope tag

Big Data in Gene Expression Profiling

Long non-coding RNA expression profiling in the NCI60 cancer cell line panel using high-throughput RT-qPCR Mestdagh P Lefever S Volders PJ Derveaux S Hellemans J Vandesompele J Sci Data 2016 Jul 3: 160052 Long non-coding RNAs (lncRNAs) form a new class of RNA molecules implicated in various aspects of protein coding gene expression

Services

In addition primary cells for example from human (blood) or from rodent (CNS) are available upon request for assay development Secondary screening for pharmacological evaluation of compound characteristics is part of our Integrated Drug Discovery expertise Mode of action biomarkers target engagement studies are provided for hit characterization and for lead finding and optimization

Gene expression profiling of human alveolar

Infection and RNA isolation Human alveolar macrophages were plated into six well culture plates at 1 10 6 cells/ml in RPMI with 2% FCS containing 50 μg/ml of gentamicin and following overnight attachment were stimulated with B anthracis spores (1 MOI) for 6 h This time was chosen based on previous experiments showing that peak cytokine mRNA induction by spores occurred between 5 and

Antibody profiling identifies novel antigenic targets in

Antibody profiling of SCI plasma samples using SAS A high-quality hSC cDNA phage display library was generated from human spinal cord tissue of 18 Caucasians Human spinal cord cDNA fragments were cloned into the pVI phage display vectors in three reading frames resulting in a total library size of 2 44 10 6 independent clones The spinal

Multiplexed enrichment and genomic profiling of

Specialized immune cell subsets are involved in autoimmune disease cancer immunity and infectious disease through a diverse range of functions mediated by overlapping pathways and signals However subset-specific responses may not be detectable in analyses of whole blood samples and no efficient approach for profiling cell subsets at high throughput from small samples is available

Simultaneous Profiling of 194 Distinct Receptor

qRT-PCR receptome profiling is accurate precise and more sensitive than oligonucleotide microarrays We defined a signaling "receptome" that includes all human receptor serine-threonine and tyrosine kinases all cytokine and chemokine receptors as well as all receptors of the Toll-like Frizzled Notch and Patched families (Fig 1A and file S1)

Reference Profiles of ExRNAs in Normal Human Pregnancy

Reference Profiles of ExRNAs in Normal Human Pregnancy Laurent Louise Chang University of California San Diego La Jolla CA United States Search 32 grants from Louise Laurent Search grants from University of California San Diego Share this grant:

STR Profiling of HTLV

We analyzed DNA from a panel of HTLV-1-infected cell lines and noninfected T-cell lines using a commercial STR kit and then analyzed the same DNA for individual STR markers followed by nondenaturing polyacrylamide gel electrophoresis This simplified method should facilitate routine confirmation of cell line identity in diverse laboratory settings Key words HTLV-1 ATLL STR profiling

Rapid isolation and profiling of a diverse panel of human

Rapid isolation and profiling of a diverse panel of human monoclonal antibodies targeting the SARS-CoV-2 spike protein immunology view on bioRxiv By Seth J Zost Pavlo Gilchuk Rita E Chen James Brett Case Joseph X Reidy Andrew Trivette Rachel S Nargi Rachel E Sutton Naveenchandra Suryadevara Elaine C Chen Elad Binshtein Swathi Shrihari Mario Ostrowski Helen Y Chu

COVID

Rapid isolation and profiling of a diverse panel of human monoclonal antibodies targeting the SARS-CoV-2 spike protein This study describes the methodology used to efficiently generate a large library of highly-functional monoclonal antibodies directed against the SARS-CoV-2 spike (S) protein using the Single Cell Immune Profiling Solution

Profiling of Accessible Chromatin Regions across Multiple

The transcriptional regulatory structure of plant genomes remains poorly defined relative to animals It is unclear how many cis -regulatory elements exist where these elements lie relative to promoters and how these features are conserved across plant species We employed the assay for transposase-accessible chromatin (ATAC-seq) in four plant species ( Arabidopsis thaliana Medicago

Isolation and Flow Cytometric Analysis of Immune Cells

The direct comparison with previously described isolation protocols 14 22 23 revealed significantly higher recovery of viable cells by Liberase TL treatment (K M unpublished data) Compared to collagenase which is frequently used for the isolation of immune cells from the brain Liberase TL contains negligible levels of endotoxin

Agilent SureSelect Human All Exon V6 V6+UTR and V6

Agilent Technologies has added the SureSelect Human All Exon V6 V6+UTR and V6+COSMIC to its line of target-enrichment products The new products are designed to address the limitations of exome sequencing targeting hard-to-capture and multi-mapping regions The products also increase the sensitivity of variant calling and minimize false negative calls Agilent said

Systemic surfaceome profiling identifies target antigens

We next assembled a diverse panel of human prostate cancer cell lines to further characterize cell-surface antigens in the PrAd and NEPC subtypes The panel included established lines including CWR22Rv1 LNCaP NE1 3 DU145 NCI-H660 and LASCPC-01 as well as two developed cell lines named NB120914 and MSKCC EF1 NB120914 was initiated from

Cell fixation and preservation for droplet

Cell preparation for droplet-based single-cell transcriptional profiling Schematic of experimental workflow Cultured human (HEK) and mouse (3T3) cells were dissociated mixed and further processed to analyse the transcriptomes of either live or fixed cells by Drop-seq Washed cells were gently resuspended in 2 volumes of ice-cold PBS then fixed by adding 8 volumes of ice-cold methanol

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